Difference between revisions of "Part:BBa K847005:Experience"
(→Applications of BBa_K847005) |
Kendrickwang (Talk | contribs) |
||
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | + | ||
| − | + | '''Assay''' <BR> | |
| + | Liquid cultures of NEB5α E. coli transformed with MntH, recA, sod Cu/Zn, Dps 1 (Sod Mn and Dps 2 constructs could not be completely assembled in time), and negative control were grown up over night at 37°C. The following day, the cells were washed and resuspended in 0.9% NaCl solution. Cell concentration was then adjusted to 10^7/mL in 5mL of a glass Petri dish. Each sample was then exposed to 1.2 J/(m^2*sec) of UV-C radiation from a UV lamp for a cumulative of 0 seconds, 2 seconds, 5 seconds, 10 seconds, 20 seconds, and 30 seconds. After each exposure, a dilution spot assay was conducted to determine the final number of surviving cells. | ||
| + | |||
| + | |||
| + | '''Results'''<BR> | ||
| + | Further testing needed. Refer to http://2012.igem.org/Team:Stanford-Brown/HellCell/Radiation. | ||
===Applications of BBa_K847005=== | ===Applications of BBa_K847005=== | ||
Revision as of 00:32, 4 October 2012
Assay
Liquid cultures of NEB5α E. coli transformed with MntH, recA, sod Cu/Zn, Dps 1 (Sod Mn and Dps 2 constructs could not be completely assembled in time), and negative control were grown up over night at 37°C. The following day, the cells were washed and resuspended in 0.9% NaCl solution. Cell concentration was then adjusted to 10^7/mL in 5mL of a glass Petri dish. Each sample was then exposed to 1.2 J/(m^2*sec) of UV-C radiation from a UV lamp for a cumulative of 0 seconds, 2 seconds, 5 seconds, 10 seconds, 20 seconds, and 30 seconds. After each exposure, a dilution spot assay was conducted to determine the final number of surviving cells.
Results
Further testing needed. Refer to http://2012.igem.org/Team:Stanford-Brown/HellCell/Radiation.
Applications of BBa_K847005
Astrobiology revolves around three central questions: "Where do we come from?", "Where are we going?", and "Are we alone?" To approach the second question, the Hell Cell subgroup of the Stanford-Brown iGEM team developed BioBricks that allow a cell to survive harsh extraterrestrial conditions. Such a toolset could create a space-ready synthetic organism to perform useful functions off-world. This gene is one of the toolset, potentially conferring radiation resistance to otherwise radiation-intolerant bacteria.
User Reviews
UNIQ2dfebfc9080fa1f0-partinfo-00000000-QINU UNIQ2dfebfc9080fa1f0-partinfo-00000001-QINU