Difference between revisions of "Part:BBa J45004:Design"
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*LENGTH: 51<br> | *LENGTH: 51<br> | ||
*GC CONTENT: 39.2 %<br> | *GC CONTENT: 39.2 %<br> | ||
| − | *MELT TEMP:<br> | + | *MELT TEMP: 65.0 ºC<br> |
| + | <br> | ||
===Source=== | ===Source=== | ||
Revision as of 15:31, 9 June 2006
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 663
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 204
Design Notes
Just CDS taken from GenBank
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 49
- GC CONTENT: 46.9 %
- MELT TEMP: 67.6 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAC -3'
- LENGTH: 51
- GC CONTENT: 39.2 %
- MELT TEMP: 65.0 ºC
Source
Species: Petunia x hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)
References
- <biblio>Underwood05 pmid=15849311</biblio>
- <biblio>Negre03 pmid=14630969</biblio>