Difference between revisions of "Part:BBa K842011"
Seancurran (Talk | contribs) (New page: Description and Function motA is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motB to form this structure, which then in tur...) |
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motA is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motB to form this structure, which then in turn controls the flagella’s rotation. | motA is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motB to form this structure, which then in turn controls the flagella’s rotation. | ||
Used for reconstitution of flagella apparatus in E. coli B strains. | Used for reconstitution of flagella apparatus in E. coli B strains. | ||
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Cloning and Uses | Cloning and Uses | ||
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motB to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway. | This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motB to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway. | ||
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Revision as of 00:18, 4 October 2012
motA is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motB to form this structure, which then in turn controls the flagella’s rotation. Used for reconstitution of flagella apparatus in E. coli B strains.
Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motB to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 323
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 61
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 829