Difference between revisions of "Part:BBa K912034"
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<partinfo>BBa_K912034 short</partinfo> | <partinfo>BBa_K912034 short</partinfo> | ||
This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector. This particular part was constructed in order to test the to make sure that the Pgad/clc system works correctly. We hoped that it would be easier to see GFP for assay purposes. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K912033 The original device] is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration. | This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector. This particular part was constructed in order to test the to make sure that the Pgad/clc system works correctly. We hoped that it would be easier to see GFP for assay purposes. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K912033 The original device] is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration. | ||
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| + | The chloride antiporter was promoted by a strong promoter. | ||
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Latest revision as of 23:38, 3 October 2012
pH Inducible GFP system (ns)
This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector. This particular part was constructed in order to test the to make sure that the Pgad/clc system works correctly. We hoped that it would be easier to see GFP for assay purposes. The original device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.
The chloride antiporter was promoted by a strong promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1992
Illegal NheI site found at 2015 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2349
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2564
Illegal AgeI site found at 3308 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1901