Difference between revisions of "Part:BBa K900000:Design"
Austin.jones (Talk | contribs) |
Austin.jones (Talk | contribs) |
||
| Line 8: | Line 8: | ||
===Source=== | ===Source=== | ||
| − | Synthesized in house. | + | Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al. (non-genomic DNA sequence). |
===References=== | ===References=== | ||
| + | |||
| + | Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009). | ||
Revision as of 22:29, 3 October 2012
Design Notes
The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.
Source
Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al. (non-genomic DNA sequence).
References
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).