Difference between revisions of "Part:BBa K842004"

Revision as of 22:02, 3 October 2012

fliC

Transcription of fliC generates the flagellin protein, which is then exported to form the flagella filament. This structure is the backbone of the flagella.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 547
Illegal PstI site found at 872
Illegal PstI site found at 919
Illegal PstI site found at 1486
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 547
Illegal PstI site found at 872
Illegal PstI site found at 919
Illegal PstI site found at 1486
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1224
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 547
Illegal PstI site found at 872
Illegal PstI site found at 919
Illegal PstI site found at 1486
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 547
Illegal PstI site found at 872
Illegal PstI site found at 919
Illegal PstI site found at 1486
Illegal AgeI site found at 301
Illegal AgeI site found at 709
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K842004 short</partinfo>
@@ Line 7: / Line 6: @@
 Used for reconstitution of flagella apparatus in E. coli B strains.
-Cloning and Uses
+Cloning and Uses:
 This part was generated via PCR from the E. coli strain DH5&#945; and cloned into the vector pSB1C3.  It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.