Difference between revisions of "Part:BBa K842003"

Revision as of 22:00, 3 October 2012

fliA

fliA is an alternate sigma factor for the class 3 flagella operons. When transcribed, it forms a component of RNA polymerase sigma 28.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3
Illegal PstI site found at 91
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3
Illegal PstI site found at 91
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3
Illegal PstI site found at 91
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3
Illegal PstI site found at 91
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 426
Illegal SapI.rc site found at 451
Illegal SapI.rc site found at 607

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K842003 short</partinfo>
@@ Line 7: / Line 6: @@
 Used for reconstitution of flagella apparatus in E. coli B strains.
-Cloning and Uses
+Cloning and Uses:
 This part was generated via PCR from the E. coli strain DH5&#945; and cloned into the vector pSB1C3.  It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.