Difference between revisions of "Part:BBa K902018"
 
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MgtAp-mgtArb-S7
 
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<html><p> This circuit was built to characterize the mgtAp-mgtArb and test how well it would function as a killswitch. S7 micrococcal nuclease was constructed with the regulatory elements and we took readings for OD600 as well as did a CFU testing. This system is repressed in the presence of magnesium and is turned on in the absence of magensium. <i>E. coli</i> uses the mgtA promoter and riboswitch combination to maintain magnesium homeostasis in the cell. This killswitch is activated in the lack of magensium. See experience page for more details.</p> </html>
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<html><p>This is the magnesium circuit with the S7 micrococal nuclease. An assay was done measure optical density of E. coli with different concentrations of magnesium.</p></html>[[Image:24 hour assay with mgtap-rb-S7.png|750px|center]]<html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:52, 3 October 2012

MgtAp-mgtArb-S7

MgtAp-mgtArb-S7

This circuit was built to characterize the mgtAp-mgtArb and test how well it would function as a killswitch. S7 micrococcal nuclease was constructed with the regulatory elements and we took readings for OD600 as well as did a CFU testing. This system is repressed in the presence of magnesium and is turned on in the absence of magensium. E. coli uses the mgtA promoter and riboswitch combination to maintain magnesium homeostasis in the cell. This killswitch is activated in the lack of magensium. See experience page for more details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]