Difference between revisions of "Part:BBa K726018"

 
 
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<partinfo>BBa_K726018 short</partinfo>
 
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This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).  
 
This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).  
  
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===Usage and Biology===
 
===Usage and Biology===
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<regulartext1> A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment. This is because the starting product of the reaction carried out by VioD and VioC is protodeoxyviolaceinic acid and is only made in the presence of VioA, VioB, and VioC. <br>
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<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioDC.jpg">
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<img align="center" style="margin-bottom:20px; width: 200px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/IMG_4881.jpg">
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However, when cells containing this construct were grown together with strains containing our VioABE construct <a href="https://parts.igem.org/Part:BBa_K726016">BBa_K726016 </a>, a purple pigment (violacein) was produced.
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More information on this process can be found on the  <a href="http://2012.igem.org/Team:UCSF/Violacein_Results">2012 UCSF iGEM Violacein Results Page </a>.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 20:57, 3 October 2012

T7Prom+HisTag+VioDC

This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).


Usage and Biology

A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment. This is because the starting product of the reaction carried out by VioD and VioC is protodeoxyviolaceinic acid and is only made in the presence of VioA, VioB, and VioC.

However, when cells containing this construct were grown together with strains containing our VioABE construct BBa_K726016 , a purple pigment (violacein) was produced. More information on this process can be found on the 2012 UCSF iGEM Violacein Results Page .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2571
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1063
    Illegal SapI.rc site found at 1138