Difference between revisions of "Part:BBa K779607"
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<partinfo>BBa_K779607 short</partinfo>
 
<partinfo>BBa_K779607 short</partinfo>
  
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This device consists of a modified TRE promoter with six tetO sites to provide low basal transcriptional activity, and the red fluroescent protein, mKate as a reporter. In the presence of rTTA3 and different concentrations of Doxycycline, we can modulate mKate expression.
  
 
https://static.igem.org/mediawiki/2012/6/68/DoxCurveGraphPad.png
 
https://static.igem.org/mediawiki/2012/6/68/DoxCurveGraphPad.png

Revision as of 20:49, 3 October 2012

Tre-Tight-mKate MammoBlock Device

This device consists of a modified TRE promoter with six tetO sites to provide low basal transcriptional activity, and the red fluroescent protein, mKate as a reporter. In the presence of rTTA3 and different concentrations of Doxycycline, we can modulate mKate expression.

DoxCurveGraphPad.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193
    Illegal BsaI.rc site found at 859
    Illegal SapI.rc site found at 241