Difference between revisions of "Part:BBa K824009"

Latest revision as of 20:02, 3 October 2012

Arsenic Promoter with GFP Reporter (Arsenic Detector)

We digested the promoter responsive to Arsenic(BBa_J33201) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 255
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1168

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 __NOTOC__
 <partinfo>BBa_K824009 short</partinfo>
-We digested the promoter responsive to Arsenic(BBa_K174015) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB3C1 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.
+We digested the promoter responsive to Arsenic(BBa_J33201) with EcoRI and SpeI and digested the GFP plasmid (BBa_I13401) with XbaI and PstI. The promoter was Chloramphenicol resistant and the GFP was Ampicillin resistant. These digested fragments were mixed and ligated to the provided, linearized pSB1C3 plasmid. The ligation mix was grown under Chloramphenicol selection. The resulting colonies were tested for responsive GFP production after the addition of Cadmium Chloride since the promoter is responsive to multiple heavy metals.
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