Difference between revisions of "Part:BBa K887004:Design"

 
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===Source===
 
===Source===
  
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The circuit is optimized from 2011 NCTU_FORMOSA.
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It is improved form NCTU-Formosa 2011 iGEM team.
  
 
===References===
 
===References===

Revision as of 18:35, 3 October 2012

Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7718
    Illegal XhoI site found at 6049
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6938
    Illegal AgeI site found at 3158
    Illegal AgeI site found at 4143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2744
    Illegal BsaI site found at 5835
    Illegal BsaI site found at 6092
    Illegal BsaI.rc site found at 836
    Illegal BsaI.rc site found at 1430
    Illegal BsaI.rc site found at 3563


Design Notes

We add zinc fingers in front of enzymes in the circuit, and therefore the enzymes could bind the DNA program in order. It help enhance the efficiency of isobutanol production .


Source

It is improved form NCTU-Formosa 2011 iGEM team.

References