Difference between revisions of "Part:BBa K842012"

(New page: Description and Function motB is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motA to form this structure, which then in tur...)
 
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Cloning and Uses
 
Cloning and Uses
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motA to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway.
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motA to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway.
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Sources
 
Sources
 
Motility protein B – Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0AF06
 
Motility protein B – Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0AF06
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K842012 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K842012 parameters</partinfo>
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Revision as of 18:08, 3 October 2012

Description and Function motB is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motA to form this structure, which then in turn controls the flagella’s rotation. It is also believed that motB is responsible for forming the structure that connects the flagella motor to the cell wall. Used for reconstitution of flagella apparatus in E. coli B strains. https://parts.igem.org/wiki/index.php?title=Part:BBa_K842010 Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motA to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway.


Sources Motility protein B – Escherichia coli (strain K12). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0AF06

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 310