Difference between revisions of "Part:BBa J45001:Experience"

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===Enzyme Activity===
 
===Enzyme Activity===
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#E. coli-expressed SAMT protein catalyzes the formation of the volatile ester methyl salicylate from salicylic acid with a Km (SA) of 83 and a Km (SAM) of 3-4. It can also methylate benzoic acid to form methyl benzoate, but its K(m) value for benzoic acid is 1720 M (much larger). Kcat/Km (SA) s-1 M-1 is 132. Activity with substrate benzoic acid is 45% relative to the activity with substrate salicylic acid. All other acids yield 0% activity.
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#E. coli-expressed SAMT protein catalyzes the formation of the volatile ester methyl salicylate from salicylic acid with a Km (SA) of 83 and a Km (SAM) of 3-4. It can also methylate benzoic acid to form methyl benzoate, but its K(m) value for benzoic acid is 1720 M (much larger). Kcat/Km for SA is 132 s-1 M-1, and activity with substrate benzoic acid is 45% of the activity with substrate salicylic acid. All other acids yield 0% activity.
  
 
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Revision as of 21:46, 8 June 2006

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Enzyme Activity

  1. E. coli-expressed SAMT protein catalyzes the formation of the volatile ester methyl salicylate from salicylic acid with a Km (SA) of 83 and a Km (SAM) of 3-4. It can also methylate benzoic acid to form methyl benzoate, but its K(m) value for benzoic acid is 1720 M (much larger). Kcat/Km for SA is 132 s-1 M-1, and activity with substrate benzoic acid is 45% of the activity with substrate salicylic acid. All other acids yield 0% activity.

UNIQ197293258d111713-partinfo-00000000-QINU UNIQ197293258d111713-partinfo-00000001-QINU