Difference between revisions of "Part:BBa K801063:Design"

Revision as of 17:59, 3 October 2012

(+)-limonene synthase 1 expression cassette for yeast

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2127
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 211
Illegal SapI.rc site found at 561

This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).

Keywords: limonen

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3'

Protein coding:

Enzymatic activity: EC-number 4.2.3.20

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

Genesequence derived from citrus limon

References

Literature references:

http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references:

@@ Line 89: / Line 89: @@
 '''Literature references:'''<br>
+*[[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002]] Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.
 <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->