Difference between revisions of "Part:BBa J45004:Design"

(→‎Design Notes)
(→‎Design Notes)
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Just CDS taken from GenBank
 
Just CDS taken from GenBank
  
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Designed Forward Primer: GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT] <br>
+
Designed Forward Strand (1 and 2):  
−
LENGTH: 48<br>
+
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG <b>ATG GAA GTT GTT GAA GTT</b> -3'<br>
−
GC CONTENT: 45.8 %<br>
+
*LENGTH: 48<br>
−
MELT TEMP: 67.7 ºC<br>
+
*GC CONTENT: 45.8 %<br>
−
<br><br>
+
*MELT TEMP: 67.7 ºC<br>
−
Designed Reverse Primer:
+
<br>
−
GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA]<br>
+
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA<b>T TAT TAA TTT ATT TTG GTC AAC</b> -3'<br>
−
LENGTH: 50<br>
+
*LENGTH: 51<br>
−
GC CONTENT: 38.0 %<br>
+
*GC CONTENT: 39.2 %<br>
−
MELT TEMP: 64.9 ºC<br>
+
*MELT TEMP:<br>
  
 
===Source===
 
===Source===

Revision as of 21:38, 8 June 2006


SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Just CDS taken from GenBank

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT -3'

  • LENGTH: 48
  • GC CONTENT: 45.8 %
  • MELT TEMP: 67.7 ºC


Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAC -3'

  • LENGTH: 51
  • GC CONTENT: 39.2 %
  • MELT TEMP:

Source

Species: Petunia x hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)

References

    • <biblio>Underwood05 pmid=15849311</biblio>
    • <biblio>Negre03 pmid=14630969</biblio>