Difference between revisions of "Part:BBa K801074:Design"

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caffeine, TEF1, expression cassette
  
 
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*Backbone name: pSB1C3'
 
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Revision as of 15:38, 3 October 2012

CaMXMT1 expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


Composite part of BBa_K319003, BBa_K801071 and BBa_K801012


Keywords: caffeine, TEF1, expression cassette


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

  • Backbone name: pSB1C3'

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBricks BBa_801073 and BBa_801075 are further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: