Difference between revisions of "Part:BBa J45001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | # | + | #changed initial stop codon to TAA TAA |
+ | |||
+ | #primer design | ||
+ | #*forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG <b>ATG ACA AAA CAA ACA CAA AAG</b> -3' | ||
+ | #*reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA<b>T TAG TTT CTT TTT GCT TG</b> -3' | ||
===Source=== | ===Source=== |
Revision as of 21:32, 8 June 2006
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 577
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 901
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 471
Illegal SapI.rc site found at 75
Design Notes
- changed initial stop codon to TAA TAA
- primer design
- forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG -3'
- reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAG TTT CTT TTT GCT TG -3'
Source
- GenBank Accession No. AF515284
- Organism = Antirrhinum Majus, Snapdragon
References
- corresponding author: Natalia Dudareva
- Pubmed: PMID: 12361714
- Abstract for SAMT enzyme:
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12361714&dopt=Abstract
- Wiki Link:
- http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6