Difference between revisions of "Part:BBa K766000:Design"

 
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<partinfo>BBa_K766000 short</partinfo>
 
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This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal.
 
This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal.
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Thank robertb from MIT!
  
 
===References===
 
===References===

Latest revision as of 15:28, 3 October 2012

AHL synthetase LuxI with His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to find a way to detect the expression and purify LuxI protein.


Source

This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal.

Thank robertb from MIT!

References