Difference between revisions of "Part:BBa K801004:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801004 short</partinfo>
 
<partinfo>BBa_K801004 short</partinfo>
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===Design Notes===
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All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.
 
All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.
  
  
  
−
===Source===
+
 
  
 
Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
 
Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
 +
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
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pTUM100, shuttle vector, yeast
 +
 +
<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
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 +
===Design Notes===
 +
 +
'''Related BioBrick:'''
 +
* [https://parts.igem.org/Part:BBa_K801000 BBa_K801000: pTUM100 yeast shuttle vector based on pYES2]
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* [https://parts.igem.org/Part:BBa_K801001 BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801002 BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter]
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* [https://parts.igem.org/Part:BBa_K801003 BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter]
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
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 +
'''Cloning details:'''<br>
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<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Quality control measures:'''<br>
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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<!--*Part was partly sequenced/Part was totally sequenced-->
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 +
'''Backbone:'''<br>
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<!--*Backbone name: pSB1C3'/?backbone_name?-->
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<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--*Copynumber: low/medium/high-->
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'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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 +
'''Enzymatic activity:'''
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<!--none/EC-number ?.?.?.?-->
 +
 +
'''Cytotoxicity:'''<br>
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<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br>
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<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
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'''Intellectual property:'''
 +
<!--Information on patent situation.-->
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<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
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<!--https://igem.org/User_Information.cgi?user_id=????/email-->
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 +
===Source===
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 +
'''Source:'''<br>
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<!--*Commercial system: plasmid name, system name, company name-->
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
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<!--*Preexisting BioBrick ?Bba_number?-->
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<!--*cDNA Clone: ?clone_name?, ?company_name?-->
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<!--*Synthesized by ?company_name?.-->
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<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
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'''Organism:'''<br>
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<!--*Genesequence derived from ''?organism_name?''-->
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
 +
  
 
===References===
 
===References===
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<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 +
 +
'''Database references:'''<br>
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<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
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<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
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<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 15:22, 3 October 2012

pTUM104 yeast shuttle vector with GAL1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5799
    Illegal suffix found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5799
    Illegal NheI site found at 3275
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 5805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5799
    Illegal XhoI site found at 5826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5799
    Illegal suffix found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5799
    Illegal XbaI site found at 5814
    Illegal NgoMIV site found at 5832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1432
    Illegal BsaI.rc site found at 2378
    Illegal SapI site found at 349
    Illegal SapI site found at 2530


All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.



Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).


Keywords: pTUM100, shuttle vector, yeast


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: