Difference between revisions of "Part:BBa K801003:Design"

Latest revision as of 15:21, 3 October 2012

pTUM103 yeast shuttle vector with pADH1 promoter

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found at 36
Illegal suffix found at 776
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 4041
Illegal SpeI site found at 777
Illegal PstI site found at 791
Illegal NotI site found at 42
Illegal NotI site found at 784
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 36
Illegal suffix found at 777
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 36
Plasmid lacks a suffix.
Illegal XbaI site found at 51
Illegal SpeI site found at 777
Illegal PstI site found at 791
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 149
Illegal BsaI.rc site found at 2198
Illegal BsaI.rc site found at 3144
Illegal SapI site found at 1115
Illegal SapI site found at 3296

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible .

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords: pADH1 promoter, pTUM100, yeast, shuttle vector

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:
Test digestion using SpeI+PstI-HF

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
pYES2, Invitrogen

Organism:
Designed for the following Chassis: Saccharomyces cerevisiae, Escherichia coli

References

Literature references:

Database references:

@@ Line 13: / Line 13: @@
 <br>'''Keywords:'''
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
+pADH1 promoter, pTUM100, yeast, shuttle vector
 <br>'''Abbreviations:'''
@@ Line 22: / Line 22: @@
 '''Related BioBrick:'''
+* [https://parts.igem.org/Part:BBa_K801000 BBa_K801000: pTUM100 yeast shuttle vector based on pYES2]
+* [https://parts.igem.org/Part:BBa_K801001 BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter]
+* [https://parts.igem.org/Part:BBa_K801002 BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter]
+* [https://parts.igem.org/Part:BBa_K801004 BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter]
 <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
-'''Cloning details:'''<br>
+'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25
 <!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
-'''Quality control measures:'''<br>
+'''Quality control measures:'''<br> Test digestion using SpeI+PstI-HF
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 <!--*Part was partly sequenced/Part was totally sequenced-->
-'''Backbone:'''<br>
+'''Backbone:'''<br> Resistance: Amp
 <!--*Backbone name: pSB1C3'/?backbone_name?-->
 <!--*Resistance: Amp/Cp/Kan/Tet-->
@@ Line 65: / Line 69: @@
 ===Source===
-'''Source:'''<br>
+'''Source:'''<br> pYES2, Invitrogen
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 75: / Line 79: @@
 <!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
-'''Organism:'''<br>
+'''Organism:'''<br>Designed for the following Chassis: ''Saccharomyces cerevisiae'', ''Escherichia coli''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===