Difference between revisions of "Part:BBa K801001:Design"

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<partinfo>BBa_K801001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801001 SequenceAndFeatures</partinfo>
  
 +
All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible
 +
 +
Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
 +
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 +
shuttle vector yeast, pTEF1
 +
 +
<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 +
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
  
 
===Design Notes===
 
===Design Notes===
−
All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible
 
  
 +
'''Related BioBrick:'''
  
 +
Other versions:
 +
* [https://parts.igem.org/Part:BBa_K801000 BBa_K801000: pTUM100 yeast shuttle vector based on pYES2]
 +
* [https://parts.igem.org/Part:BBa_K801002 BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801003 BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801004 BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter]
 +
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 +
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 +
 +
'''Cloning details:'''<br> Designed in RFC10/RFC23/RFC25
 +
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 +
 +
'''Quality control measures:'''<br> Test digestion using SpeI+ PstI
 +
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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<!--*Part was partly sequenced/Part was totally sequenced-->
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 +
'''Backbone:'''<br> Resistance: Amp
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<!--*Backbone name: pSB1C3'/?backbone_name?-->
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<!--*Resistance: Amp/Cp/Kan/Tet-->
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<!--*Copynumber: low/medium/high-->
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 +
'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 +
 +
'''Enzymatic activity:'''
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<!--none/EC-number ?.?.?.?-->
 +
 +
'''Cytotoxicity:'''<br>
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<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br>
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<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
 +
 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
 +
<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).
+
'''Source:'''<br> Commercial system: pYES2, Invitrogen
 +
<!--*Commercial system: plasmid name, system name, company name-->
 +
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 +
<!--*Preexisting BioBrick ?Bba_number?-->
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<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
 +
 
 +
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 
 +
'''Organism:'''<br> Designed for the following Chassis:''Saccharomyces cerevisiae'', ''Escherichia coli''
 +
<!--*Genesequence derived from ''?organism_name?''-->
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<!--*Codonoptimized for ''?organism_name?''-->
 +
<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 +
 +
'''Database references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
 +
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 +
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 15:20, 3 October 2012

pTUM101 yeast shuttle vector with pTEF1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3771
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
    Illegal NotI site found at 42
    Illegal NotI site found at 514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 261
    Illegal BsaI.rc site found at 1928
    Illegal BsaI.rc site found at 2874
    Illegal SapI site found at 845
    Illegal SapI site found at 3026

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).


Keywords: shuttle vector yeast, pTEF1


Abbreviations:

Design Notes

Related BioBrick:

Other versions:

Quality control measures:
Test digestion using SpeI+ PstI

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2, Invitrogen


Organism:
Designed for the following Chassis:Saccharomyces cerevisiae, Escherichia coli

References

Literature references:

Database references: