Difference between revisions of "Part:BBa K801092:Design"

Latest revision as of 12:14, 3 October 2012

4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1092
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1659
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1291

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/609339/

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase

Abbreviations: 4CL = 4-coumarate-coenzyme A Ligase

Design Notes

Related BioBrick:

Other versions: BBa_K801093: 4CL coding region

Related BioBricks:

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:
Protein: 4-coumarate-coenzyme A ligase

Enzymatic activity:
EC-number: 6.2.1.12

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Arabidopsis thaliana

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 GenBank: Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q42524 Uniprot: 4-coumarate--CoA ligase 1]

@@ Line 11: / Line 11: @@
 PMID: 15932991
 <br>'''Keywords:'''
 Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase
@@ Line 20: / Line 21: @@
 '''Related BioBrick:'''
-<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
-<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
+Other versions: [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4CL coding region]
+Related BioBricks:
+* [https://parts.igem.org/Part:BBa_K801090 BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801091 BBa_K801091: phenylalanine ammonia lyase (PAL) coding region]
+* [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region]
+* [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: Naringenin - chalcone synthase (CHS) coding region]
+* [https://parts.igem.org/Part:BBa_K801096 BBa_K801096: Aromatic prenyltransferase (APT) coding region]
+* [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence]
+* [https://parts.igem.org/Part:BBa_K801098 BBa_K801098: O-methyltransferase 1 (OMT1) coding region]
 '''Cloning details:'''<br>
-<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
+Designed in RFC10/RFC25
 <!--*Mutation C889G to delete XbaI restriction site-->
 <!--*Truncation upstream/downstream compared to template, ?explanation?-->
@@ Line 34: / Line 45: @@
 '''Backbone:'''<br>
-Backbone name: pSB1C3
+*Backbone name: pSB1C3
-Resistance: Cp
+*Resistance: Cp
-<!--*Copynumber: low/medium/high-->
+*Copynumber: high
 '''Protein coding:'''<br>
@@ Line 44: / Line 55: @@
 <!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
-'''Enzymatic activity:'''
+'''Enzymatic activity:'''<br>
 EC-number: 6.2.1.12
@@ Line 65: / Line 76: @@
 '''Source:'''<br>
 <!--*Commercial system: plasmid name, system name, company name-->
-*Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA
+Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA
 <!--*Preexisting BioBrick ?Bba_number?-->
 <!--*cDNA Clone: ?clone_name?, ?company_name?-->
@@ Line 78: / Line 89: @@
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
@@ Line 89: / Line 99: @@
 '''Database references:'''<br>
 [http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 '''GenBank''': Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]
 <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
-[http://www.uniprot.org/uniprot/Q42524  '''Uniprot''': 4-coumarate--CoA ligase 1]-->
+[http://www.uniprot.org/uniprot/Q42524  '''Uniprot''': 4-coumarate--CoA ligase 1]
 <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 <!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->