Difference between revisions of "Part:BBa K801092:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801092 short</partinfo>
 
<partinfo>BBa_K801092 short</partinfo>
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<partinfo>BBa_K801092 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801092 SequenceAndFeatures</partinfo>
  
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Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
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http://www.ncbi.nlm.nih.gov/nucleotide/609339/
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PMID: 15932991
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<br>'''Keywords:'''
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Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase
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<br>'''Abbreviations:'''
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4CL = 4-coumarate-coenzyme A Ligase
  
 
===Design Notes===
 
===Design Notes===
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-
 
  
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'''Related BioBrick:'''
  
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Other versions: [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4CL coding region]
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Related BioBricks:
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* [https://parts.igem.org/Part:BBa_K801090 BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence]
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* [https://parts.igem.org/Part:BBa_K801091 BBa_K801091: phenylalanine ammonia lyase (PAL) coding region]
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* [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region]
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* [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence]
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* [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: Naringenin - chalcone synthase (CHS) coding region]
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* [https://parts.igem.org/Part:BBa_K801096 BBa_K801096: Aromatic prenyltransferase (APT) coding region]
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* [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence]
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* [https://parts.igem.org/Part:BBa_K801098 BBa_K801098: O-methyltransferase 1 (OMT1) coding region]
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'''Cloning details:'''<br>
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Designed in RFC10/RFC25
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<!--*Mutation C889G to delete XbaI restriction site-->
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<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Quality control measures:'''<br>
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<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
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<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
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Part was totally sequenced
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'''Backbone:'''<br>
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*Backbone name: pSB1C3
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*Resistance: Cp
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*Copynumber: high
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'''Protein coding:'''<br>
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Protein: 4-coumarate-coenzyme A ligase
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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'''Enzymatic activity:'''<br>
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EC-number: 6.2.1.12
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'''Cytotoxicity:'''<br>
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<!--none/not known/cytotoxic for ''organism name''-->
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'''Safety notes:'''<br>
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<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
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<!--Known and anticipated security issues: none/other.-->
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'''Intellectual property:'''
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<!--Information on patent situation.-->
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<!--Intellectual property claims made by the authors.-->
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'''Corresponding part author/authors:'''
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<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
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Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
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'''Source:'''<br>
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<!--*Commercial system: plasmid name, system name, company name-->
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Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA
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<!--*Preexisting BioBrick ?Bba_number?-->
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<!--*cDNA Clone: ?clone_name?, ?company_name?-->
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<!--*Synthesized by ?company_name?.-->
  
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http://www.ncbi.nlm.nih.gov/nucleotide/609339/
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<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
  
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PMID: 15932991
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'''Organism:'''<br>
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Genesequence derived from ''Arabidopsis thaliana''
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<!--*Codonoptimized for ''?organism_name?''-->
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<!--*Designed for the following Chassis: ''?organism-name?''-->
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<!--*Statement about functionality in other chassis.-->
  
 
===References===
 
===References===
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<!-- Here you find templates to insert references to literature and different databases-->
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'''Literature references:'''<br>
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[http://www.ncbi.nlm.nih.gov/pubmed/15932991 '''Pubmed''' : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]
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'''Database references:'''<br>
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[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 '''GenBank''': Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]
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<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
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[http://www.uniprot.org/uniprot/Q42524  '''Uniprot''': 4-coumarate--CoA ligase 1]
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<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
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<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
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<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Latest revision as of 12:14, 3 October 2012

4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1291


Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/609339/

PMID: 15932991


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase


Abbreviations: 4CL = 4-coumarate-coenzyme A Ligase

Design Notes

Related BioBrick:

Other versions: BBa_K801093: 4CL coding region

Related BioBricks:

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:
Protein: 4-coumarate-coenzyme A ligase

Enzymatic activity:
EC-number: 6.2.1.12

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:
Genesequence derived from Arabidopsis thaliana

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 GenBank: Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q42524 Uniprot: 4-coumarate--CoA ligase 1]