Difference between revisions of "Part:BBa K801073"
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Yeast expression cassette for CaXMT1 ([https://parts.igem.org/Part:BBa_K801070 BBa_K801070]) controlled by the yeast TEF2 promoter ([https://parts.igem.org/Part:BBa_K801010 BBa_K801010]) and the yeast ADH1 terminator ([https://parts.igem.org/Part:BBa_K801012 BBa_K801012]). | Yeast expression cassette for CaXMT1 ([https://parts.igem.org/Part:BBa_K801070 BBa_K801070]) controlled by the yeast TEF2 promoter ([https://parts.igem.org/Part:BBa_K801010 BBa_K801010]) and the yeast ADH1 terminator ([https://parts.igem.org/Part:BBa_K801012 BBa_K801012]). | ||
| − | This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. For creating an expression cassette with all three enzymes of the caffeine synthesis pathway based on the substrate Xanthosine [https://parts.igem.org/Part:BBa_K801077 BBa_K801077] different promoters and terminators were assembled to each enzyme. CaXMT1 is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants ( | + | This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. For creating an expression cassette with all three enzymes of the caffeine synthesis pathway based on the substrate Xanthosine [https://parts.igem.org/Part:BBa_K801077 BBa_K801077] different promoters and terminators were assembled to each enzyme. CaXMT1 is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (with regard to genome integration). |
Revision as of 12:05, 3 October 2012
CaXMT1 expression cassette for yeast
Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).
This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. For creating an expression cassette with all three enzymes of the caffeine synthesis pathway based on the substrate Xanthosine BBa_K801077 different promoters and terminators were assembled to each enzyme. CaXMT1 is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (with regard to genome integration).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 138
- 1000COMPATIBLE WITH RFC[1000]