Difference between revisions of "Part:BBa J45004:Design"

Revision as of 21:11, 8 June 2006

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 663
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 204

Design Notes

Just CDS taken from GenBank

Designed Forward Primer:
GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT]
LENGTH: 48
GC CONTENT: 45.8 %
MELT TEMP: 67.7 ºC

Designed Reverse Primer: GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA]
LENGTH: 50
GC CONTENT: 38.0 %
MELT TEMP: 64.9 ºC

Source

Species: Petunia x hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)

References

- <biblio>Underwood05 pmid=15849311</biblio>
- <biblio>Negre03 pmid=14630969</biblio>

@@ Line 9: / Line 9: @@
 Just CDS taken from GenBank
-Designed Forward Primer:
+Designed Forward Primer: <Br>
-GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT]
+GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT] <br>
-LENGTH:	48
+LENGTH:	48<br>
-GC CONTENT:	45.8 %
+GC CONTENT:	45.8 %<br>
-MELT TEMP:	67.7 ºC
+MELT TEMP:	67.7 ºC<br>
-MOLECULAR
+<br><br>
-WEIGHT:	14850.6 g/mole
+Designed Reverse Primer:
-EXTINCTION
+GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA]<br>
-COEFFICIENT:	455100 L/(mole·cm)
+LENGTH:	50<br>
-nmole/OD260:	2.20
+GC CONTENT:	38.0 %<br>
-µg/OD260:	32.63
+MELT TEMP:	64.9 ºC<br>
 ===Source===