Difference between revisions of "Part:BBa K801073"

Revision as of 11:53, 3 October 2012

CaXMT1 expression cassette for yeast

Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).

This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K801073 short</partinfo>
-Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).
+Yeast expression cassette for CaXMT1 ([https://parts.igem.org/Part:BBa_K801070 BBa_K801070]) controlled by the yeast TEF2 promoter ([https://parts.igem.org/Part:BBa_K801010 BBa_K801010]) and the yeast ADH1 terminator ([https://parts.igem.org/Part:BBa_K801012 BBa_K801012]).
-This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1)and part of the caffeine synthesis pathway. It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator is Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).
+This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).