Difference between revisions of "Part:BBa K801004:Design"
(→‎Design Notes)
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
 +
* [https://parts.igem.org/Part:BBa_K801000 BBa_K801000: pTUM100 yeast shuttle vector based on pYES2]
 +
* [https://parts.igem.org/Part:BBa_K801001 BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801002 BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801003 BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter]
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]

Revision as of 11:53, 3 October 2012

pTUM104 yeast shuttle vector with GAL1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5799
    Illegal suffix found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5799
    Illegal NheI site found at 3275
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 5805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5799
    Illegal XhoI site found at 5826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5799
    Illegal suffix found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5799
    Illegal XbaI site found at 5814
    Illegal NgoMIV site found at 5832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1432
    Illegal BsaI.rc site found at 2378
    Illegal SapI site found at 349
    Illegal SapI site found at 2530


All forbidden restriction sites have been removed, making this plasmid RFC25-compatible.



Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: