Difference between revisions of "Part:BBa K801002:Design"
(→‎Design Notes)
 
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<br>'''Keywords:'''
 
<br>'''Keywords:'''
 +
* pTUM, pYES2, TEF2-Promoter
 
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 
<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
 
<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
  
 
<br>'''Abbreviations:'''
 
<br>'''Abbreviations:'''
 +
* TEF2
 
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
 
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
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===Design Notes===
 
===Design Notes===
  
−
'''Related BioBrick:'''
+
'''Related BioBrick:'''  
 +
* [https://parts.igem.org/Part:BBa_K801000 BBa_K801000: pTUM100 yeast shuttle vector based on pYES2]
 +
* [https://parts.igem.org/Part:BBa_K801001 BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801003 BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter]
 +
* [https://parts.igem.org/Part:BBa_K801004 BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter]
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
 
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
−
'''Cloning details:'''<br>
+
'''Cloning details:'''<br> Designed in RFC10
−
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
+
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->  
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
  
−
'''Quality control measures:'''<br>
+
'''Quality control measures:'''<br> Testdigestion using XbaI+PstI-HF, and EcorI-HF+SpeI-HF
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
 
<!--*Part was partly sequenced/Part was totally sequenced-->
  
−
'''Backbone:'''<br>
+
'''Backbone:'''<br> Resistance: Amp
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Copynumber: low/medium/high-->
 
<!--*Copynumber: low/medium/high-->
  
−
'''Protein coding:'''<br>
+
'''Protein coding:'''<br>  
 
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
 
<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
Line 46: Line 52:
 
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
  
−
'''Enzymatic activity:'''
+
'''Enzymatic activity:''' none
 
<!--none/EC-number ?.?.?.?-->
 
<!--none/EC-number ?.?.?.?-->
  
Line 52: Line 58:
 
<!--none/not known/cytotoxic for ''organism name''-->
 
<!--none/not known/cytotoxic for ''organism name''-->
  
−
'''Safety notes:'''<br>
+
'''Safety notes:'''<br> health_risk
 
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 
<!--Known and anticipated security issues: none/other.-->
 
<!--Known and anticipated security issues: none/other.-->
  
−
'''Intellectual property:'''
+
'''Intellectual property:'''  
−
<!--Information on patent situation.-->
+
<!--Information on patent situation.-->  
 
<!--Intellectual property claims made by the authors.-->
 
<!--Intellectual property claims made by the authors.-->
  
−
'''Corresponding part author/authors:'''
+
'''Corresponding part author/authors:'''  
 
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
 
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
'''Source:'''<br>
+
'''Source:'''<br> pYES2, pYES2 Vector kit, company: Invitrogen
−
<!--*Commercial system: plasmid name, system name, company name-->
+
 
 +
<!--*Commercial system: plasmid name, system name, company name-->  
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 
<!--*Preexisting BioBrick ?Bba_number?-->
 
<!--*Preexisting BioBrick ?Bba_number?-->
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<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
  
−
'''Organism:'''<br>
+
'''Organism:'''<br> Designed for E.coli, Yeast
 
<!--*Genesequence derived from ''?organism_name?''-->
 
<!--*Genesequence derived from ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->

Latest revision as of 11:50, 3 October 2012

pTUM102 yeast shuttle vector with pTEF2 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3970
    Illegal SpeI site found at 706
    Illegal PstI site found at 720
    Illegal NotI site found at 42
    Illegal NotI site found at 713
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 706
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 706
    Illegal PstI site found at 720
    Illegal NgoMIV site found at 194
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2127
    Illegal BsaI.rc site found at 3073
    Illegal SapI site found at 1044
    Illegal SapI site found at 3225


All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.


Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).


Keywords:

  • pTUM, pYES2, TEF2-Promoter


Abbreviations:

  • TEF2

Design Notes

Related BioBrick:

Quality control measures:
Testdigestion using XbaI+PstI-HF, and EcorI-HF+SpeI-HF

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity: none

Cytotoxicity:

Safety notes:
health_risk

Intellectual property:

Corresponding part author/authors:

Source

Source:
pYES2, pYES2 Vector kit, company: Invitrogen


Organism:
Designed for E.coli, Yeast


References

Literature references:

Database references: