Difference between revisions of "Part:BBa K801000:Design"
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Other versions:[https://parts.igem.org/Part:BBa_K801001 BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter], [https://parts.igem.org/Part:BBa_K801002 BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter], [https://parts.igem.org/Part:BBa_K801003 BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter], [https://parts.igem.org/Part:BBa_K801004 BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter]
 
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Revision as of 11:41, 3 October 2012

pTUM100 yeast shuttle vector based on pYES2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3379
    Illegal SpeI site found at 115
    Illegal PstI site found at 129
    Illegal NotI site found at 42
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal XhoI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Illegal suffix found at 105
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 69
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 79
    Illegal BsaI.rc site found at 1536
    Illegal BsaI.rc site found at 2482
    Illegal SapI site found at 453
    Illegal SapI site found at 2634



Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Other versions:BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter, BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter, BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter, BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: