Difference between revisions of "Part:BBa P1010"

Revision as of 20:52, 8 June 2006

ccdB cell death gene

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die.

BioBrick Plasmids Containing This Part

Most BioBrick plasmids are delivered with the P1010 insert see Physical DNA for the current list of plasmids that are available in this form.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 664
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 114

@@ Line 10: / Line 10: @@
 |}
-P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert
+P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die.
-see [https://parts.igem.org/cgi/partsdb/dna.cgi?part_name=P1010 Physical DNA] for the current list of plasmids that are available in this form.
 ===BioBrick Plasmids Containing This Part===
+Most BioBrick plasmids are delivered with the P1010 insert
+see [https://parts.igem.org/cgi/partsdb/dna.cgi?part_name=P1010 Physical DNA] for the current list of plasmids that are available in this form.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_P1010 SequenceAndFeatures</partinfo>