Difference between revisions of "Part:BBa P1010"

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P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert
 
P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert
see [https://parts.igem.org/cgi/partsdb/dna.cgi Physical DNA] for the current list of plasmids that are available in this form.
+
see [https://parts.igem.org/cgi/partsdb/dna.cgi?part_name=P1010 Physical DNA] for the current list of plasmids that are available in this form.
  
 
===BioBrick Plasmids Containing This Part===
 
===BioBrick Plasmids Containing This Part===

Revision as of 20:44, 8 June 2006

ccdB cell death gene

CcdB Plasmid.png

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert see Physical DNA for the current list of plasmids that are available in this form.

BioBrick Plasmids Containing This Part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114