Difference between revisions of "Part:BBa K741017"
 
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<partinfo>BBa_K741017 short</partinfo>
 
<partinfo>BBa_K741017 short</partinfo>
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The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited.
 
  
 
The fusion protein crogfp is consistently being produced. When the promoter plac is activated by IPTG, protein CI will be expressed to activate pRM. Then antisense RNA of cro will be expressed to inhibit the expression of CroGFP.This part is used to measure the function of pRM-anticro.
 
The fusion protein crogfp is consistently being produced. When the promoter plac is activated by IPTG, protein CI will be expressed to activate pRM. Then antisense RNA of cro will be expressed to inhibit the expression of CroGFP.This part is used to measure the function of pRM-anticro.

Latest revision as of 07:59, 3 October 2012

plac-RBS-cI-T-pRM-anticro-T-pCon(0.244)-RBS-crogfp-T

The fusion protein crogfp is consistently being produced. When the promoter plac is activated by IPTG, protein CI will be expressed to activate pRM. Then antisense RNA of cro will be expressed to inhibit the expression of CroGFP.This part is used to measure the function of pRM-anticro.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1589
    Illegal NheI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1370
    Illegal BglII site found at 1706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2500