Difference between revisions of "Part:BBa K902068:Design"
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<partinfo>BBa_K902068 short</partinfo> | <partinfo>BBa_K902068 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | This serves as regulatory element which activates expression of the pBAD operon on the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902065 pRha rhamnose promoter]. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993). | |
+ | In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR]-rhamnose complex (Egan & Schlief, 1993). | ||
+ | Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details. | ||
===Source=== | ===Source=== |
Revision as of 07:40, 3 October 2012
RhaS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 172
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This serves as regulatory element which activates expression of the pBAD operon on the pRha rhamnose promoter. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).
In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).
Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.
Source
Amplified