Difference between revisions of "Part:BBa K847210"
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<partinfo>BBa_K847210 short</partinfo> | <partinfo>BBa_K847210 short</partinfo> | ||
+ | The nrd promoter (nrdP) is a cell-division dependent promoter. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The nrd operon in E. coli expresses ribonucleotide reductase, an enzyme that reduces ribonucleotides into deoxyribonucleotides and is involved in bacterial cell cycle (Sun 1994). The promoter region is highly regulated and contains several DnaA boxes, indicating that activity is partly affected by initiation of DNA replication (Messer 2002). The promoter for this operon was found to begin activation during initiation of DNA replication and is cell-cycle dependent (Sun 1992). | |
+ | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K847210 SequenceAndFeatures</partinfo> | <partinfo>BBa_K847210 SequenceAndFeatures</partinfo> |
Revision as of 07:34, 3 October 2012
polA (DNA Polymerase I) DnaA-activated promoter
The nrd promoter (nrdP) is a cell-division dependent promoter.
Usage and Biology
The nrd operon in E. coli expresses ribonucleotide reductase, an enzyme that reduces ribonucleotides into deoxyribonucleotides and is involved in bacterial cell cycle (Sun 1994). The promoter region is highly regulated and contains several DnaA boxes, indicating that activity is partly affected by initiation of DNA replication (Messer 2002). The promoter for this operon was found to begin activation during initiation of DNA replication and is cell-cycle dependent (Sun 1992).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 144
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]