Difference between revisions of "Part:BBa K902065:Design"

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The pRha promoter is two promoters in one. In its native E. coli, the 5' end of the promoter serves to express the rhaR and rhaS control genes. The 3' end of the promoter is expresses the rhaBAD rhamnose metabolism genes.  
 
The pRha promoter is two promoters in one. In its native E. coli, the 5' end of the promoter serves to express the rhaR and rhaS control genes. The 3' end of the promoter is expresses the rhaBAD rhamnose metabolism genes.  
  
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The rhamnose response: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR] transcription factor is activated by rhamnose to upregulate [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 rhaR] and rhaS[https://parts.igem.org/wiki/index.php?title=Part:BBa_K902068 rhaS]. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902068 RhaS] in turn up-regulates the rhaBAD operon. These functions are dependent on the binding of the catabolite receptor protein (CRP) cAMP complex to the promoter. Additionally, function of RhaS is improved in the presence of rhamnose (Egan & Schleif, 1993).
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The rhamnose response: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 RhaR] transcription factor is activated by rhamnose to upregulate [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902069 rhaR] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902068 rhaS]. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K902068 RhaS] in turn up-regulates the rhaBAD operon. These functions are dependent on the binding of the catabolite receptor protein (CRP) cAMP complex to the promoter. Additionally, function of RhaS is improved in the presence of rhamnose (Egan & Schleif, 1993).
  
 
The glucose response: The promoter is subject to repression via global catabolite repression.
 
The glucose response: The promoter is subject to repression via global catabolite repression.

Revision as of 07:25, 3 October 2012

Rhamnose inducible, glucose repressible promoter (pRha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pRha promoter is two promoters in one. In its native E. coli, the 5' end of the promoter serves to express the rhaR and rhaS control genes. The 3' end of the promoter is expresses the rhaBAD rhamnose metabolism genes.

The rhamnose response: RhaR transcription factor is activated by rhamnose to upregulate rhaR and rhaS. RhaS in turn up-regulates the rhaBAD operon. These functions are dependent on the binding of the catabolite receptor protein (CRP) cAMP complex to the promoter. Additionally, function of RhaS is improved in the presence of rhamnose (Egan & Schleif, 1993).

The glucose response: The promoter is subject to repression via global catabolite repression.

Please see the details on our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki].

Source

This promoter was commercially synthesized from the sequence characterized by Jeske and Altenbuchner (2010).

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.

Jeske, M., & Altenbuchner, J. (2010). The escherichia coli rhamnose promoter rhaP BAD is in pseudomonas putida KT2440 independent of crp--cAMP activation. Applied Microbiology and Biotechnology, 85(6), 1923-1933.