Difference between revisions of "Part:BBa K778001"
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''E.coli'' overexpressing FhlA produce H<sub>2</sub> (since hycAp is the promoter of FHL(formate hydrogen lyase)). | ''E.coli'' overexpressing FhlA produce H<sub>2</sub> (since hycAp is the promoter of FHL(formate hydrogen lyase)). | ||
− | [[Image:UT Tokyo H2 1001.png]] | + | [[Image:UT Tokyo H2 1001+cm.png]] |
(The full construct in this experiment is [[Part:BBa K778007]] and | (The full construct in this experiment is [[Part:BBa K778007]] and |
Revision as of 05:33, 3 October 2012
fhlA: a transcriptional activator for enhanced hydrogen production
FhlA is the transcriptional activator of hycAp Part:BBa K778000 .
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
(The full construct in this experiment is Part:BBa K778007 and The modified fhlA(E363G) part is Part:BBa K778002.)
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2096
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1375
- 1000COMPATIBLE WITH RFC[1000]