Difference between revisions of "Part:BBa P1010"
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P1010 is used in BioBrick assembly aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
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P1010 is used to put BioBrick parts
  
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aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up.
  
 
===BioBrick Plasmids Containing This Part===
 
===BioBrick Plasmids Containing This Part===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_P1010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_P1010 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_P1010 parameters</partinfo>
 
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Revision as of 20:15, 8 June 2006

ccdB cell death gene

CcdB Plasmid.png

The CcdB protein, expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used to put BioBrick parts

aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up.

BioBrick Plasmids Containing This Part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114