Difference between revisions of "Part:BBa K902048:Design"
 
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===Design Notes===
 
===Design Notes===
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===References===
 
===References===
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<sup>1</sup>Shu L, Chiou Y, Orville AM, Miller MA, Lipscomb JD, Que L. X-ray absorption spectroscopic studies of the Fe(II) active site of catechol 2,3-dioxygenase. Implications for the extradiol cleavage mechanism. Biochem 1995; 34:6649-6659.

Latest revision as of 04:32, 3 October 2012

Constitutive XylE generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 571
    Illegal AgeI site found at 922
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Both the tetR promoter and the XylE gene were from the 2012 kit plates.

References

1Shu L, Chiou Y, Orville AM, Miller MA, Lipscomb JD, Que L. X-ray absorption spectroscopic studies of the Fe(II) active site of catechol 2,3-dioxygenase. Implications for the extradiol cleavage mechanism. Biochem 1995; 34:6649-6659.