Difference between revisions of "Part:BBa K799009"

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<partinfo>BBa_K799009 short</partinfo>
 
<partinfo>BBa_K799009 short</partinfo>
  
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____ promoter shows an increase in protein expression when exposed to __% ethanol.
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YMR251W-A promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.
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We tested this by expressing GFP under the control of YMR251W-A promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.
  
 
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===Plasmid Map===
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===Characterization===
  
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[[]]
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[[Image:ProA5 fluoro.png]]
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This is fluorescence data for the expression of GFP under the control of YMR251W-A, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
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===Plasmid Map===
  
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[[Image:YMR251W-A plasmid.png]]
  
 
===Sequencing===
 
===Sequencing===

Latest revision as of 04:15, 3 October 2012

Ethanol/Stress Inducible YMR251W-A Promoter

YMR251W-A promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.

We tested this by expressing GFP under the control of YMR251W-A promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 120
    Illegal PstI site found at 277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 169
    Illegal NheI site found at 355
    Illegal PstI site found at 277
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 120
    Illegal PstI site found at 277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 120
    Illegal PstI site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

ProA5 fluoro.png

This is fluorescence data for the expression of GFP under the control of YMR251W-A, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YMR251W-A plasmid.png

Sequencing

ProA5 sequencing.png

This is the sequencing result for YMR251W-A. As can be seen, it is a perfect match to the genomic DNA promoter.