Difference between revisions of "Part:BBa K799005"
 
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<partinfo>BBa_K799005 short</partinfo>
 
<partinfo>BBa_K799005 short</partinfo>
  
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YML070W promoter shows an increase in protein expression when exposed to up to 14% ethanol.
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YML070W promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.
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We tested this by expressing GFP under the control of YML070W promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.
  
 
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[[Image:ProA3 fluoro.png]]
 
[[Image:ProA3 fluoro.png]]
  
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This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
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This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
  
 
===Plasmid Map===
 
===Plasmid Map===
  
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[[]]
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[[Image:YML070W plasmid.png]]
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===Sequencing===
 
===Sequencing===
  
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[[]]
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[[Image:YML070W sequencing.png]]
  
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This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.
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This is the sequencing result for the YML070W promoter. As can be seen, it is a perfect match to the genomic DNA promoter.

Latest revision as of 04:12, 3 October 2012

Ethanol/Stress Inducible YML070W Promoter

YML070W promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.

We tested this by expressing GFP under the control of YML070W promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA3 fluoro.png

This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YML070W plasmid.png

Sequencing

YML070W sequencing.png

This is the sequencing result for the YML070W promoter. As can be seen, it is a perfect match to the genomic DNA promoter.