Difference between revisions of "Part:BBa K799003"
 
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<partinfo>BBa_K799003 short</partinfo>
 
<partinfo>BBa_K799003 short</partinfo>
  
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____ promoter shows an increase in protein expression when exposed to __% ethanol.
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YJL052W promoter shows an increase in protein expression when exposed to up to 14% ethanol.
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We tested this by expressing GFP under the control of YJL052W promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.
  
 
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<partinfo>BBa_K799003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K799003 SequenceAndFeatures</partinfo>
  
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===Characterization===
  
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===Plasmid Map===
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[[Image:ProA2 fluoro.png]]
  
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[[]]
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This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
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===Plasmid Map===
  
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[[Image:YJL052W plasmid.png]]
  
 
===Sequencing===
 
===Sequencing===
  
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[[]]
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[[Image:ProA2 sequencing.png]]
  
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This is the sequencing result for colony 1. Colony 1 is a perfect match to the genomic DNA promoter.
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This is the sequencing result for promoter YJL052W. As can be seen, it is a perfect match to the genomic DNA promoter.

Latest revision as of 04:11, 3 October 2012

Ethanol/Stress Inducible YJL052W Promoter

YJL052W promoter shows an increase in protein expression when exposed to up to 14% ethanol.

We tested this by expressing GFP under the control of YJL052W promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA2 fluoro.png

This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YJL052W plasmid.png

Sequencing

ProA2 sequencing.png

This is the sequencing result for promoter YJL052W. As can be seen, it is a perfect match to the genomic DNA promoter.