Difference between revisions of "Part:BBa K799001"
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| − | YJL200C promoter | + | YJL200C promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol. |
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| + | We tested this by expressing GFP under the control of YJL200C promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP. | ||
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===Plasmid Map=== | ===Plasmid Map=== | ||
| − | [[]] | + | [[Image:YJL200C plasmid.png]] |
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===Sequencing=== | ===Sequencing=== | ||
Latest revision as of 04:08, 3 October 2012
Ethanol/Stress Inducible YJL200C Promoter
YJL200C promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.
We tested this by expressing GFP under the control of YJL200C promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 172
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 172
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 172
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 172
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
Plasmid Map
Sequencing
This is the sequencing result for YJL200C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.