Difference between revisions of "Part:BBa K799001"

 
 
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<partinfo>BBa_K799001 short</partinfo>
 
<partinfo>BBa_K799001 short</partinfo>
  
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Shows protein expression when induced with ethanol.
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YJL200C promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.
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We tested this by expressing GFP under the control of YJL200C promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.  
  
 
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<partinfo>BBa_K799001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K799001 SequenceAndFeatures</partinfo>
  
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===Characterization===
  
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[[Image:ProA1 fluoro.png]]
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===Functional Parameters===
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<partinfo>BBa_K799001 parameters</partinfo>
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This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
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===Plasmid Map===
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[[Image:YJL200C plasmid.png]]
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===Sequencing===
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[[Image:YJL200C sequencing.png]]
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This is the sequencing result for YJL200C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.

Latest revision as of 04:08, 3 October 2012

Ethanol/Stress Inducible YJL200C Promoter

YJL200C promoter is expected to drive gene expression in Saccharomyces cerevisiae in the presence of ethanol.

We tested this by expressing GFP under the control of YJL200C promoter in the presence of multiple different concentrations of ethanol. We found an induction of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 172
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 172
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA1 fluoro.png

This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

YJL200C plasmid.png

Sequencing

YJL200C sequencing.png

This is the sequencing result for YJL200C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.