Difference between revisions of "Part:BBa P1010"
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The CcdB protein, expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant. | The CcdB protein, expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant. | ||
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Revision as of 20:06, 8 June 2006
ccdB cell death gene
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The CcdB protein, expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant. |
P1010 is used in BioBrick assembly aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.
This is the part that is present inside the BioBricks insertion site in many of the pSB series of plasmids. It is sufficient for positive selection even in pSB2* series plasmids in the absence of inducer (~4-5 copies per cell).
During assembly work at the Registry, we have found that point mutations (particularly frame shifts) occur at a high enough rate that several colonies will survive during a normal transformation. -Randy
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 664
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 114