Difference between revisions of "Part:BBa K799029"
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<partinfo>BBa_K799029 short</partinfo> | <partinfo>BBa_K799029 short</partinfo> | ||
| − | Terminator of the MFA2 gene from Saccharomyces | + | Terminator of the MFA2 gene from ''Saccharomyces cerevisiae''. It confers relative instability to the mRNA produced, thus reducing the amount of mRNA retained in the cell and possibly reducing the production of protein. https://www.jstage.jst.go.jp/article/bbb/75/11/75_110246/_pdf "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System" |
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[[Image:MFA2t sequence.png]] | [[Image:MFA2t sequence.png]] | ||
| − | This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the | + | This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the expected sequence. |
Latest revision as of 03:43, 3 October 2012
MFA2 Terminator
Terminator of the MFA2 gene from Saccharomyces cerevisiae. It confers relative instability to the mRNA produced, thus reducing the amount of mRNA retained in the cell and possibly reducing the production of protein. https://www.jstage.jst.go.jp/article/bbb/75/11/75_110246/_pdf "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 217
Plasmid Map
Sequencing
This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the expected sequence.