Difference between revisions of "Part:BBa K799029"

Latest revision as of 03:43, 3 October 2012

MFA2 Terminator

Terminator of the MFA2 gene from Saccharomyces cerevisiae. It confers relative instability to the mRNA produced, thus reducing the amount of mRNA retained in the cell and possibly reducing the production of protein. https://www.jstage.jst.go.jp/article/bbb/75/11/75_110246/_pdf "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 217

Plasmid Map

Sequencing

This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the expected sequence.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K799029 short</partinfo>
-Terminator of the MFA2 gene.
+Terminator of the MFA2 gene from ''Saccharomyces cerevisiae''. It confers relative instability to the mRNA produced, thus reducing the amount of mRNA retained in the cell and possibly reducing the production of protein. https://www.jstage.jst.go.jp/article/bbb/75/11/75_110246/_pdf "TPS1 Terminator Increases mRNA and Protein Yield in a Saccharomyces Cerevisiae Expression System"
 <!-- Add more about the biology of this part here
@@ Line 13: / Line 13: @@
 ===Plasmid Map===
-[[]]
+[[Image:MFA2t plasmid.png]]
 ===Sequencing===
@@ Line 20: / Line 19: @@
 [[Image:MFA2t sequence.png]]
-This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the genomic DNA terminator.
+This is the sequencing result for the MFA2 gene terminator. As can be seen, it is a perfect match to the expected sequence.