Difference between revisions of "Part:BBa J45002:Design"

(→‎Design Notes)
(→‎Design Notes)
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*Bases 1-18 of the original ''A. majus'' sequence (gccggacgccaaagaaaa) were deleted.  Thus, this sequence begins with the first start codon.
 
*Bases 1-18 of the original ''A. majus'' sequence (gccggacgccaaagaaaa) were deleted.  Thus, this sequence begins with the first start codon.
 +
*A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
  
 
===Source===
 
===Source===

Revision as of 19:49, 8 June 2006


SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:

<biblio>

  1. Dudareva pmid=11051108

</biblio>

  • Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
  • A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.

Source

Genbank accession number(s): AF198492

We got this cDNA from N Dudareva at Purdue.

References