Difference between revisions of "Part:BBa K902050"
 
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<partinfo>BBa_K902050 short</partinfo>
 
<partinfo>BBa_K902050 short</partinfo>
  
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<html>A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH<sub>2</sub> is consumed by this reaction.<br>
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<html>A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH<sub>2</sub> is consumed by this reaction, and therefore when this protein is employed a flavin mononucleotide oxidoreductase should also be used (such as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902057">BBa_K902057</a> or <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K804006">BBa_K804006</a>)<br>
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The functional DszA protein is dimeric</html>
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</html>[[Image:Ucalgary2012-DszApathway partspage.PNG]]
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</html>[[Image:Ucalgary2012-DszApathway partspage.PNG|centre]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 02:16, 3 October 2012

DBT-sulfone monooxygenase (DszA) from R. erythropolis

A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction, and therefore when this protein is employed a flavin mononucleotide oxidoreductase should also be used (such as BBa_K902057 or BBa_K804006)
Ucalgary2012-DszApathway partspage.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 682
    Illegal AgeI site found at 217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1066