Difference between revisions of "Part:BBa J45013:Design"
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Orum H and Rasmussen OF. Expression in E. coli of the gene encoding phenylalanine ammonia-lyase from Rhodosporidium toruloides. Appl Microbiol Biotechnol 1992 Mar; 36(6) 745-7. pmid:1368015. PubMed HubMed [orum92] | Orum H and Rasmussen OF. Expression in E. coli of the gene encoding phenylalanine ammonia-lyase from Rhodosporidium toruloides. Appl Microbiol Biotechnol 1992 Mar; 36(6) 745-7. pmid:1368015. PubMed HubMed [orum92] | ||
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Rasmussen OF and Oerum H. Analysis of the gene for phenylalanine ammonia-lyase from Rhodosporidium toruloides. DNA Seq 1991; 1(3) 207-11. pmid:1773059. PubMed HubMed [rasmussen91] | Rasmussen OF and Oerum H. Analysis of the gene for phenylalanine ammonia-lyase from Rhodosporidium toruloides. DNA Seq 1991; 1(3) 207-11. pmid:1773059. PubMed HubMed [rasmussen91] | ||
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Faulkner JD, Anson JG, Tuite MF, and Minton NP. High-level expression of the phenylalanine ammonia lyase-encoding gene from Rhodosporidium toruloides in Saccharomyces cerevisiae and Escherichia coli using a bifunctional expression system. Gene 1994 May 27; 143(1) 13-20. pmid:8200528. PubMed HubMed [faulkner94] | Faulkner JD, Anson JG, Tuite MF, and Minton NP. High-level expression of the phenylalanine ammonia lyase-encoding gene from Rhodosporidium toruloides in Saccharomyces cerevisiae and Escherichia coli using a bifunctional expression system. Gene 1994 May 27; 143(1) 13-20. pmid:8200528. PubMed HubMed [faulkner94] | ||
Latest revision as of 19:49, 8 June 2006
PAL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2090
Illegal BamHI site found at 352
Illegal XhoI site found at 403
Illegal XhoI site found at 466
Illegal XhoI site found at 484
Illegal XhoI site found at 562
Illegal XhoI site found at 763
Illegal XhoI site found at 1006
Illegal XhoI site found at 1753 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 922
Illegal NgoMIV site found at 1168
Illegal NgoMIV site found at 1336
Illegal NgoMIV site found at 1479
Illegal NgoMIV site found at 1813 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 463
Illegal BsaI site found at 793
Illegal BsaI site found at 799
Illegal BsaI.rc site found at 1924
Illegal SapI site found at 753
Design Notes
The purpose of this part would be to develop cinnamic acid from natural food for E. coli. From there, if we can find the SAM:CCMT part, we could develop a cinnamon smell from E. coli merely from the cells eating their natural food.
Source
If we received such a part, it would probably be donated by Faulkner, et al. If not, the part would need to be modified to successfully express it in E. coli. GENBANK Accession Number = X12702
References
Orum H and Rasmussen OF. Expression in E. coli of the gene encoding phenylalanine ammonia-lyase from Rhodosporidium toruloides. Appl Microbiol Biotechnol 1992 Mar; 36(6) 745-7. pmid:1368015. PubMed HubMed [orum92]
Rasmussen OF and Oerum H. Analysis of the gene for phenylalanine ammonia-lyase from Rhodosporidium toruloides. DNA Seq 1991; 1(3) 207-11. pmid:1773059. PubMed HubMed [rasmussen91]
Faulkner JD, Anson JG, Tuite MF, and Minton NP. High-level expression of the phenylalanine ammonia lyase-encoding gene from Rhodosporidium toruloides in Saccharomyces cerevisiae and Escherichia coli using a bifunctional expression system. Gene 1994 May 27; 143(1) 13-20. pmid:8200528. PubMed HubMed [faulkner94]